Our data show that it is possible to transduce rat endothelial cells with hHO-1 gene and to achieve selective hHO-1 expression to levels significantly higher than those of endogenous rat HO-1 expression. The overexpression of hHO-1 in rat endothelial cells was associated with an elevation in cGMP. Our data also reveal that augmentation of hHO-1 activity in transduced cells conferred substantial protection against cellular toxicity produced by H2O2 and heme.
CiteULike: Retrovirus-mediated HO gene transfer into endothelial cells protects against oxidant-induced injury: "Yang, L., Quan, S., and Abraham, N. G. (1999). Retrovirus-mediated ho gene transfer into endothelial cells protects against oxidant-induced injury. Am J Physiol Lung Cell Mol Physiol, 277(1):L127-133."
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